Aim: The aim of this study is to specifically determine the effectiveness of its bactericidal activity in different concentrations against the causative agent of periodontitis, Aggregatibacter actinomycetemcomitants.
Study design: Experimental method of research was used in this study. In the experimental study, the researchers manipulated five (5) concentrations of potassium alum, 100 percent (100%), eighty percent (80%), sixty percent (60%), forty percent (40%), and twenty percent (20%). The Kirby-Bauer disk diffusion method is the main tool in determining the effectiveness of potassium alum in various concentrations against A. actinomycetemcomitants.
Place and Duration of Study: Department of Medical Technology Laboratory, University of Perpetual Help-Dr. Jose G. Tamayo Medical University, between November 2016 and October 2017.
Methodology: The test organism ysed was A. actinomycetemcomitants. The organism was contained in an ampoule and as it is opened, 0.3mL to 0.5 mL of Nutrient broth was added into the ampoule using a sterile pasteur pipette. This served as the re-hydration solution for the bacteria. Sample was streaked on the prepared BAP. Blood agars with the bacteria were placed in candle jars and incubated at 37 degrees Celsius for 24 to 48 hours. Preparing the bacteria for susceptibility was done by selecting several colonies from the cultured plate of A. actinomycetemcomitans and suspending it in Nutrient broth adjusting it equal to a McFarlans 0.5 standard. Then 100%, 80%, 60%, 40% and 20% concentrations of potassium alum were used. The prepared alum solution was used for testing its effectiveness in reducing A. actinomycetemcomitans. Afterwards, the measurement was erad and interpreted with the aid of a Zone Diameter interpretive chart. The researchers used amoxicillin as the basis for the measurement of zone of inhibition.
Results: All of the concentrations of potassium alum can inhibit the growth of A. actinomycetemcomitans. However, 100% and 80% concentrations being the most effective that falls under the interpretation Susceptible, yielding a 19 mm and 18 mm, respectively. The researchers used ANOVA test in their study. The value of the test statistic F=76.917, greater than the critical value of F=2.848, and the P value is <0.001, Therefore, null hypothesis is rejected. Furthermore, this means that there is a significant difference between the zones of inhibition of all the concentrations and the zone of inhibition of amoxicillin.
Conclusion: The researchers therefore concluded that all concentrations of potassium alum can effectively reduce A. actinomycetemcomitans. Results indicated positive outcomes but it is recommended for further experimentation. It is also highly recommended for future researchers to use densitometer for more accurate and consistent bacterial suspension to be used in Kirby Bauer Diffusion Method.